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Bacillus thuringiensis (Bt) in Brief

This fact sheet describes Bt used as a pesticide in sprays, granules, and other products. Bt crops are different. They're called "genetically engineered" or "transgenic" crops because they use DNA from Bt bacteria. The plants make Bt proteins (toxins) that only target specific insects.1 The Bt toxin made by the plant is called a "plant-incorporated protectant."

What is Bt?

tomato hornworm, photo credit: pam carter, pixabay

Bt is a bacterium that is found naturally in soils throughout the world. To reproduce, Bt makes spores that grow into new bacteria. Bt spores have proteins that are toxic to insect larvae when eaten.2 Because Bt comes from a natural source, it is called a biopesticide. In general, biopesticides tend to pose fewer risks than typical human-made pesticides.

There are many types of Bt. Each type or strain affects different insect groups. Target insects include the beetle family, the fly family including mosquitoes, and the butterfly family. It is used to kill beetles and the larvae of mosquitoes, black flies, and moths.2,3,4 Bt is also toxic to nematodes.5

Bt has been registered for use in pesticides by the U.S. Environmental Protection Agency (U.S. EPA) since 1961. The U.S. EPA requires routine testing to ensure that unwanted toxins and bacteria are not present.2 Some of these unwanted toxins may be harmful to non-target animals and people.6

What are some products that contain Bt?

Currently, Bt is found in over 130 registered pesticide products.7 Bt products are used on crops and ornamental plants. They are used in greenhouses, non-residential buildings, in aquatic settings, and in aerial applications. Common products include sprays, dusts, granules, concentrates, and pellets.8 Some of these products are approved for use in organic agriculture.9

Technical Grade Bt: This fact sheet refers to the spores of Bt and the toxins the spores release when insects eat them. Products you buy from the store include other ingredients in addition to Bt. While many of the other ingredients may not pose health or environmental risks, some of them can be toxic. The other ingredients can pose greater risks than the Bt itself.

IMPORTANT: Always follow label instructions and take steps to minimize exposure. If any exposures occur, be sure to follow the First Aid instructions on the product label carefully. For additional treatment advice, contact the Poison Control Center at 800-222-1222. If you wish to discuss a pesticide problem, please call the National Pesticide Information Center at 800-858-7378.

How does Bt work?

Spores made by Bt damage the gut of insect larvae after the larvae eat them.2 The insect gut must have a pH of 9.0 to 10.5 (high pH) in order to activate the toxin.2,10 This is different from the human gut, which has a low pH and is more acidic. The activated toxin breaks down the insect's gut lining. The insect larva dies of infection and starvation.10,11,12 Death occurs within 1-5 days.10,12 Young insect larvae are most affected.1

Each type of Bt toxin is specific to the target insect family.2,3 Some strains of Bt toxins are also toxic to nematodes.1

mosquito larvae, photo credit: James Gathany, CDC

Common types of Bt strains:

Bt toxins are not activated when the spores are eaten by people, and no harm occurs.2 The toxins are destroyed by acidic conditions such as those in the human stomach.13 People and other mammals do not have the specific enzymes that break down the spore proteins to release the toxins. Mammals also do not have the necessary receptors that insect guts have.1,2 In human stomachs, the toxin proteins are easily digested.14

How might I be exposed to Bt?

What are pesticide tolerances?

The EPA sets legal limits for how much pesticide is allowed in food and drinking water. In food, those limits are called "tolerances." Most pesticides have their own tolerance for each crop it can be used on. Some pesticides that are very low in toxicity do not require a tolerance and are "exempt." In water, those limits may be called Maximum Contaminant Levels (MCLs), health advisories (HA), or other names. The amount allowed in water is specifically regulated for some pesticides. Health advisories are issued for others.

You can be exposed to Bt if you breathe it in or get it on your skin or eyes. For example, this can occur while applying sprays or dusts during windy conditions. You may also be exposed after using a product if you don't wash your hands before eating, drinking, or smoking. Because Bt is commonly found in soils, exposures not related to pesticides are also possible.3 Bt has also been found in drinking water. The source may have been from mosquito spraying, use of other pesticide products containing Bt, or from naturally occurring Bt.15

Pets and people might be exposed to Bt from treated birdbaths or water fountains. You can limit exposure and reduce risk by carefully following the label instructions.

Bt is used on many crops, and you may be exposed to very low levels of Bt through your diet.2 The U.S. EPA sets maximum limits for pesticide residues on food. Unlike most other pesticides, Bt is exempt from this requirement because of its low toxicity. See the text box about pesticide tolerances.

What are some signs and symptoms from a brief exposure to Bt?

Effects in laboratory animals:

Bt is very low in toxicity to people and other mammals when inhaled.2,8

Bt is low in toxicity to people and other mammals when eaten.2,8

Effects in people:

Pesticide Risk:

Any chemical, including any pesticide, can pose risks to people, pets, or the environment. Understanding pesticide risk will help you take steps to minimize it. The risk of a pesticide depends on two things, exposure (how much?) and toxicity (how poisonous?). The exposure is the amount you get in or on your body, or the amount that is released into the environment. The toxicity of a pesticide is a measure of how poisonous it is to people or the environment. Even products that are low in toxicity can be hazardous if the exposure is high enough. Take steps to lower your chance of exposure to reduce your risk.

There is little evidence of sickness or infection in people as a result of exposure to Bt.6,14,17 However, some pesticide products with Bt in them have caused eye and skin irritation.2

TOXICITY CLASSIFICATION - Bacillus thuringiensis2 (see the text box about mg/kg)
High Toxicity Moderate Toxicity Low Toxicity Very Low Toxicity
Acute Oral LD50 ≤ 50 mg/kg > 50-500 mg/kg > 500-5000 mg/kg >5000 mg/kg
Inhalation LC50 ≤ 0.05 mg/L > 0.05-0.5 mg/L > 0.5-2.0 mg/L > 2.0 mg/L
Dermal LD50 ≤ 200 mg/kg > 200-2000 mg/kg > 2000-5000 mg/kg > 5000 mg/kg
Primary Eye Irritation* Corrosive (irreversible destruction of ocular tissue) or corneal involvement or irritation persisting for more than 21 days Corneal involvement or other eye irritation clearing in 8 - 21 days Corneal involvement or other eye irritation clearing in 7 days or less Minimal effects clearing in less than 24 hours
Primary Skin Irritation* Corrosive (tissue destruction into the dermis and/or scarring) Severe irritation at 72 hours (severe erythema or edema) Moderate irritation at 72 hours (moderate erythema) Mild or slight irritation at 72 hours (no irritation or erythema)

*The U.S. EPA did not require eye or skin irritation tests for pure Bt. Some tests with purchase-ready products resulted in slight to moderate skin or eye irritation. These tests included both Bt and other ingredients. Irritation may have been caused by the other ingredients.

The shaded boxes reflect signs and symptoms observed after a brief exposure. Modeled after the U.S. Environmental Protection Agency, Office of Pesticide Programs, Label Review Manual, Chapter 7: Precautionary Statements. https://www.epa.gov/sites/default/files/2018-04/documents/chap-07-mar-2018.pdf. Data from EPA 1998, Reregistration Eligibility Decision (RED) for Bacillus thuringiensis.

You may be wondering why the "High Toxicity" column has smaller numbers than the "Low Toxicity" column. This is because if a smaller amount of the pesticide caused a health effect, it's more toxic. If it takes a larger amount of the pesticide to cause a health effect, it's less toxic.

Scientists also evaluated whether Bt can cause allergic reactions.

What happens to Bt when it enters the body?

When eaten, Bt is confined to the gut. Bt leaves the body in as little as 2 days.6 Neither Bt nor the toxic proteins are stored in fat.24

If breathed in, Bt can move to the lungs, blood, lymph, and kidneys. Levels of Bt decrease quickly one day after exposure.6

Rats that inhaled Bt israelensis spores for 30 minutes had spores present in their lungs afterwards for 3 days. The spores disappeared by seven days after exposure. Scientists concluded that the spores remained in the lungs.25 The presence of spores does not mean that there is an infection.14

Is Bt likely to contribute to the development of cancer?

No data were found on the carcinogenic (cancer causing) effects of Bt in humans or animals.

The toxins produced by Bt are proteins. Research has shown that eating Bt proteins has not led to cancer, genetic mutations, or birth defects.26

Has anyone studied non-cancer effects from long-term exposure to Bt?

Long-term feeding studies in rats showed few negative effects. Scientists used doses of 1.3 billion spores of Bt kurstaki per kg of body weight per day for 13 weeks, and 8,400 mg/kg of Bt kurstaki for 90 days. See the text box about mg/kg. The rats showed no signs of illness. Female rats fed 8,400 mg/kg Bt kurstaki for two years gained less weight than the rats who did not eat Bt, but none of the rats showed signs of toxicity or that they were infected.6

Are children more sensitive to Bt than adults?

Young children may act in ways that put them at greater risk of being exposed. For example, they may spend more time near or on the floor. They may also be more likely to place their hands in their mouths after touching treated surfaces or pets.

The U.S. EPA concluded that "risk is not expected" to children or infants from eating food treated with Bt.2 Children living in an area sprayed with Bt for gypsy moths did not have more asthma or lower lung function compared to children outside the spray area. The researchers used peak expiratory flow rate to measure lung function.21

What happens to Bt in the environment?

Naturally occurring Bt spores have been found in soil, on plants, and in dead insects.27 Spores may spread from insect droppings and from the bacteria growing in dead insects that were not killed by Bt.28


Bt spores in soil may break down more quickly or slowly depending on the conditions. Bt toxins generally break down much faster than the spores, with half-lives of less than 1 day to 46 days. However, some toxin may remain in soil for up to six months.29

Rain, plant growth, and presence of invertebrates in natural soil do not seem to affect naturally occurring Bt. However, Bt may pass through the digestive tracts of animals and multiply in their feces. This may help Bt spread in the environment.30

Additional details about how Bt breaks down in soil:

Bt spores usually remain in the top several inches of soil.32 Bt may remain dormant in most natural soil conditions.34 Whether Bt spores survive and grow in soil depends on soil properties.30


On leaf surfaces, Bt is broken down by the sun's UV light, weather, and enzymes. These naturally occurring plant enzymes speed up the breakdown of proteins on the leaf. Spores may wash off the leaf surface as well.1 Typical foliage half-lives are 1 to 4 days.2

In one study, the half-life of Bt spores on soybean leaves was less than one day. After one day, less than 9% of the spores were still able to grow into new Bt cells.3 The spores break down rapidly on plant surfaces because of UV light.2

Bt from the soil can settle on plant leaves naturally.28 It can be splashed by rainwater from soil to leaves, or can be deposited by animal feces, sprouting seeds, or infected insects.30

Researchers found that cotton and cabbage seedlings took up Bt kurstaki through their roots. The Bt was then found throughout the plants. The plants' leaves were then toxic to moth caterpillars. Bt was also found in cotton plants that grew in an area that had never been treated with Bt.36


Bt is not expected to reproduce or multiply in water.2 However, Bt has been found in many aquatic environments. Bt may reach natural waters from rain, wind, animal feces, and runoff.30


Sunlight breaks down Bt spores quickly. In one study, over 80% could no longer grow within one hour of sun exposure.3,37

Can Bt affect birds, fish, or other wildlife?

Terrestrial animals

Bt is practically nontoxic and doesn't cause disease in birds, fish, and shrimp. No negative effects or infection was found in rats fed large doses of Bt strains or forced to inhale the spores.6 It does not cause disease in mammals.3 There is no evidence that Bt can cause a disease outbreak among wild animals.2,8

potato beetle, photo credit: pavlofox, pixabay

Bt is considered practically nontoxic to bobwhite quail and mallard ducks that were fed doses of 3,100 mg/kg (3.1 g/kg) each day for five days. No mallards died after ingesting a single dose of 10 g/kg Bt. Bt was also practically nontoxic to birds when inhaled. The LC50 was greater than 8,570 mg/kg. Scientists tested Bt tenebrionis, israelensis, aizawai, and kurstaki strains.2

Scientists have observed little to no direct toxicity to non-target insects and other arthropods. Spraying Bt kurstaki for spruce budworm did not affect soil-dwelling organisms.38

Bt strains had little to no toxicity to lacewings, predatory beetles, parasitic wasps, or earthworms when these were not targets of the strain.2 Bt does not seem to hurt earthworms, although it has been found in their gut contents.32 Other scientists found that Bt did not harm earthworms when used according to typical label instructions. However, high doses of the bacteria caused fatal infection in the earthworms.39 In addition, a few studies also found that non-target moths were harmed.40

How naturally occurring Bt behaves in its environment is not yet well understood.30,39,41 Bt has been found in nematodes, soil crustaceans, and earthworms.39 Naturally occurring Bt can kill nematodes. It is also spread by nematodes.5 Naturally occurring Bt may be taken up by plant roots and translocated throughout the plant, making the leaves toxic to moth caterpillars.36

Bees and other pollinators

Several studies have tested formulated products with Bt for bee toxicity. These studies did not account for the presence of other ingredients besides Bt in the products.42,43,44 Some of these studies found effects on bumblebee and honeybee survival and behavior when the bees were exposed to products containing Bt aizawai, but these effects could have been from the other ingredients in the products. More research is needed. See the text box about Technical Grade Bt.

Scientists tested the toxicity of pure Bt aizawai to honeybees. They found that the 72-hour LD50 of the pure Bt aizawai strain was greater than 100 μg/bee. This is considered practically nontoxic.45 The EPA also concluded that the Bt strains tenebrionis, israelensis, and kurstaki are low in toxicity to bees.2

Aquatic life

Nearly all the studies that looked at whether Bt may harm non-target aquatic animals found no effects. Some species of stoneflies, mayflies, and caddisflies were negatively affected in some studies but not others.40

Scientists found that Bt kurstaki, israelensis, tenebrionis, and aizawai strains were practically nontoxic to trout, bluegill sunfish, and sheepshead minnows. The LC50 values ranged from 8.7 to 49 billion cfu/L (colony-forming units per liter).2 Scientists exposed young brook trout to concentrations of a formulated product containing Bt israelensis for 45 minutes. The brook trout did not seem to be affected until they were exposed to concentrations of 3 g/L or more of the product.46

Researchers exposed the tadpoles of European common frogs to a formulated product containing Bt israelensis at measured field concentrations of 1 mg/L. They also exposed tadpoles to 10 mg/L and 100 mg/L. The Bt product was sprinkled on the water six days apart. The researchers saw no effects on the tadpoles' development or overall health.47

Other scientists performed a similar experiment with the same species of frog. They exposed the tadpoles to three formulated products at three concentrations per product three times during the experiment. The smaller exposures mimicked those that would occur in the environment. The researchers considered the largest dose as a worst case. The exposed tadpoles showed evidence that detoxification processes in their bodies had occurred. The tadpoles had higher neuronal enzyme (AchE) activity during the first two exposures to all products at all concentrations. However, tadpole weight and survival were not affected.48

Water fleas (Daphnia) exposed to the kurstaki and israelensis strains showed moderate toxicity. The aizawai strains seemed to be highly toxic to water fleas. However, evidence suggested that toxicity in those tests may have been related to impurities from the production of Bt.2

Adult zebrafish were exposed to four of the toxins produced by Bt kurstaki at a concentration of 100 mg/L for 96 hours. The fish showed no sign of toxicity. However, zebrafish embryos and larvae exposed to the toxins showed developmental delays and toxic responses after being exposed for 96 hours to concentrations of 25, 50, 100, and 150 mg/L. The different toxins affected the fish differently.49

fly larvae, photo credit: EM80, pixabay

Bt strains including aizawai were practically nontoxic to grass shrimp, sheepshead minnows, or copepods, which live in estuaries or salt water.2 Copepods are small relatives of crabs, shrimp, and barnacles.

Scientists looked at whether a single use of Bt may harm non-target aquatic insects or fish when Bt was applied to a river. They found no effects.50 Some studies did find some impacts when products were used as the labels directed in aquatic systems. Many of the affected species were close relatives of black flies and mosquitoes. Some scientists have found that repeated applications of Bt may affect non-target organisms indirectly by affecting the food web.40,51

Where can I get more information?

For more detailed information about Bacillus thuringiensis (Bt) please visit the referenced resources below or call NPIC, Monday - Friday, between 8:00am - 12:00pm Pacific Time (11:00am - 3:00pm Eastern Time) at 800-858-7378.

Date Reviewed: May 2022

Please cite as: Gervais, J.; Cocks, M.; Cross, A.; Jenkins, J. 2022. Bacillus thuringiensis (Bt) Fact Sheet; National Pesticide Information Center, Oregon State University Extension Services. http://npic.orst.edu/factsheets/btgen.html.


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NPIC fact sheets are designed to answer questions that are commonly asked by the general public about pesticides that are regulated by the U.S. Environmental Protection Agency (U.S. EPA). This document is intended to be educational in nature and helpful to consumers for making decisions about pesticide use.